DETECTION OF mecA and seh GENES FROM Staphylococcus sp. ISOLATED FROM SAMPLES OF FOOD, SURFACES AND UTENSILS OF AN INDUSTRIAL KITCHEN IN RIO DE JANEIRO
DOI:
https://doi.org/10.12957/demetra.2017.26426Keywords:
Staphylococcus. Food Contamination. Mass Spectrometry. Methicillin resistance. Bacterial ToxinsAbstract
Staphylococci are microorganisms increasingly associated with food poisoning. This work aimed to determine the prevalence, and to detect factors of virulence and resistance, of Staphylococcus spp. isolated from samples of food, utensils and handling surfaces in an industrial kitchen of a supermarket chain in Rio de Janeiro; and to analyze the presence of genes encoding staphylococcal enterotoxin H (seh) and antimicrobial resistance (mecA). A total of 50 samples were collected between January and March 2016. After isolation, microbial species were identified by MALDI-TOF MS, as well as seh and mecA genes, which were detected by polymerase chain reaction (PCR). Of the 50 samples analyzed, 45 (90%) were positive for Staphylococcus sp. Presence; 41 samples were of the genus, 37 of which were identified at the species level, 40 samples coagulase-negative Staphylococcus (CNS), and one coagulase-positive (CPS). The identification of the species by the MALDI-TOF MS technique proved to be accurate. The seh gene was detected in only one CNS sample (S. saprophyticus), isolated from a cold slicer. When the PCR technique was performed for the detection of the mecA gene in the 41 strains obtained from Staphylococcus sp., 6 food samples and 7 surface samples were positive. It is concluded that CNSs are widespread in the environment and need to be given more attention with regard to their detection in food.
DOI: 10.12957/demetra.2017.26426
Downloads
Downloads
Published
How to Cite
Issue
Section
License
STATEMENT OF AUTHORSHIP RESPONSIBILITY
Title of the manuscript:
________________________________________________________
1. Statement of responsability
I certify that I have participated in the work above specified and take public responsibility for its content.
I certify that the manuscript represents an original work and that none of the material in the manuscript has been previously published, is included in another manuscript, or is currently under consideration for publication elsewhere, whether in printed form or in electronic media, except that described in the attachment.
In case of acceptance of this text by Demetra: Alimentação, Nutrição & Saude, I declare to be in accordance with the policy of public access and copyright adopted by Demetra, which provides as follows: (a) the authors retain the copyright and grant to the Journal the right of first publication, the work being simultaneously licensed under the Creative Commons Attribution License, which allows the sharing of the work with acknowledgment of authorship and initial publication in this journal; (b) authors are authorized to enter additional contracts separately for non-exclusive distribution of the version of the work published in this journal (eg, publishing in institutional repository or book chapter), with acknowledgment of authorship and initial publication in this journal; and (c) authors are permitted and encouraged to post and distribute their work online (eg, in institutional repositories or on their personal page) at any point before or during the editorial process, as this may lead to productive changes, as well as increase the impact and citation of the published work.
2. Conflict of Interest Statement
I certify that there is no conflict of interest in connection with the submitted article.
Date, signature and full address of all authors.
