THE “PROTEIN DIET” REDUCES GLOMERULAR DIAMETER AND VOLUME DENSITY IN RATS
DOI:
https://doi.org/10.12957/demetra.2016.18380Keywords:
Kidney. Diet. Kidney Glomerulus. Dietary Proteins.Abstract
Objective: This study aimed to evaluate the effect of a protein diet on kidney morphology. Methods: Forty adult Rattus Norvegicus Wistar albino were divided into four groups (n = 10): Control 1 (C1) Control 2 (C2), High Protein 1 (HP1) and High Protein 2 (HP2). The C2 and HP2 groups were subjected to 30% of food restriction. At the end of the experiment, kidneys were removed, weighed and their length and width, measured. Thereafter, they were cleaved according to the Orientator method, fixed in formalin and embedded in paraffin. The blades of the histological sections were stained according to the Hematoxylin & Eosin technique and photomicrographed. Glomerular density was determined by the stereological method and the diameter of renal glomeruli was calculated using Image J software. Serum analyses were performed according to the colorimetric method. Results are expressed as mean and standard deviation. To compare the means between groups, it was used ANOVA and Tukey as post-test, accepting a significance level of 5%. Results: The HP groups had higher blood pressure. The kidney size and weight of HP1 was greater than C1 and C2. The HP1 group showed lower glomerular diameter and the HP2 group showed lower glomerular density than C1 and C2 groups. Conclusion: The "protein diet" can cause increased systolic blood pressure, abnormal renal morphology, reduced glomerular diameter and glomerular volume density. This study suggests that this diet can lead to changes in kidney tissue, predisposing individuals to development of chronic diseases.
DOI: 10.12957/demetra.2016.18380
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