Antinociceptive and anti-inflammatory activities of the hexanic extract of "Echinodorus macrophyllus" (Kunth) Micheli in mice
DOI:
https://doi.org/10.12957/bjhbs.2019.53056Abstract
Introduction: Echinodorus macrophyllus (Kunth) Micheli,
Alismataceae, commonly known as “chapéu de couro”, is
used in the treatment of various inflammatory conditions.
The aim of this study was to evaluate the antinociceptive
and anti-inflammatory neurogenic potential and perform the
phytochemical analysis of its hexanic extract (HEEm). Material
and methods: The HEEm was obtained by maceration of
dried leaves with hexane (100 g d.w./2 L). Its composition was
determined by GC-MS (DB1 column) by comparison of retention
indices in the database and literature. The antinociceptive
potential was evaluated in SW or DBA/1 male mice using
chemical (acetic acid and formalin), thermal (tail immersion
and hot plate tests) and topical (xylene) nociception models,
all approved by the Ethics Committee (CEA-IBRAG). Results:
HEEM presented antinociceptive activity in the model of:
acetic acid-induced writhing (52%; 25mg/kg); tail immersion
(60 and 90 minutes; 50 mg/kg); hot-plate in 60 minutes (25
and 100mg/kg) and 120 minutes (25mg/kg); formalin tests, at
the neurogenic (63.4%, 100mg/kg), and inflammatory (50%;
50 and 100mg/kg) phases; and in neurogenic inflammation
induced by xylene (88.3%; 100mg/kg). These activities seem to
be related to the terpene and fatty acid derivatives evidenced
by GC-MS. Discussion: HEEm presented antinociceptive, as
well as anti-inflammatory, activity by central and peripheral
mechanisms, It consists of terpenic and fatty acid derivatives,
described in the literature as antioxidants, anti-inflammatory,
and antinociceptives. Conclusions: HEEm showed antinociceptive
activity in all models, which can be related to the
presence of terpenic and fatty acid derivatives.
Keywords: Echinodorus macrophyllus; Nociception;
Neurogenic inflammation; Phytochemistry.
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